Folic acid, which provides the transfer of single carbon atoms in synthesis reactions and metabolic cycles in metabolism, is very important for metabolism. Folic acid also plays an important role in nucleotide synthesis and methylation reactions. There are many disorders caused by defected folic acid metabolism and lack of folic acid. Today, innovative, cost-effective methods are needed to develop folic acid determination method. The main objective of this study is the synthesis and characterization of folic acid imprinted nanostructures (FA-Imp-poly(MPTS-rGO-co-NAT), which will be used for the first time for folic acid determination in commercially human blood plasma. For this purpose, the synthesis of nanostructures has been carried out and characterized by FTIR, SEM-EDS, and AFM. Then, new chemically modified nanosensor was fabricated for determination of folic acid using folic acid imprinted nanostructures. Differential pulse voltametry (DPV) and circular voltametry (CV) methods were used as electrochemical methods in the FA-imprinted-nanosensor studies. Measurements in differential pulse voltametry were performed at an application speed of 0.005 volts per second in the potential range of -0.4 to 0.6 volts. As a result of the circular voltametric method, an idea about the surface was obtained with the voltamograms obtained. The detection limit (LOD) of the developed FA-imprinted-nanosensor was 7.54 ng/mL and the determination limit (LOQ) was 25.14 ng/mL. 10 and 20 ng/mL FA analytical was added to commercial synthetic serum samples by standard adding method and RSD values of 0.092 and 0.734% were found in DPV technique and measurements respectively. This study demonstrated a novel, simple, selective and rapid FA-imprinted-nanosensor for determining the FA in the biological samples.

KEYWORDS: Folic acid, nanostructures, MIP, nanobiosensors