Does high molecular weight-hyaluronic acid prevent hormone-induced preterm labor in rats?


CİLAKER MIÇILI S., Tarı O., Neri I., Proietti S., Unfer V.

European review for medical and pharmacological sciences, vol.27, no.7, pp.3022-3032, 2023 (SCI-Expanded) identifier identifier identifier

Abstract

OBJECTIVE: The aim of our study was to test if oral high hyaluronic acid (HMW-HA) administration was effective in contrasting induced preterm birth (PTB) in female Wistar rats. MATERIALS AND METHODS: A total of 24 pregnant rats were pretreated with placebo or low (2.5 mg/day)/high dose (5 mg/day) of HMW-HA (day 15) and then induced to delivery with mifepristone plus prostaglandin E2 (PGE2) (3 mg/100 µL + 0.5 mg/animal) on the 19th day of pregnancy. The delivery time was recorded and the messenger RNA (mRNA) levels of pro-inflammatory cytokines [tumor necrosis factor-α (TNF-α), interleukin (IL)1β, IL-6] were detected in the uterine tissues by real-time polymerase chain reaction (real PCR). Immunohistochemistry was performed alongside. RESULTS: Oral HMW-HA was well absorbed in the body and was able to significantly delay the timing of delivery and decrease mRNA synthesis of pro-inflammatory cytokines. CONCLUSIONS: HMW-HA, by acting in the management of PTB, may represent a new approach to protecting physiological pregnancy.