Comparison of the roles of bilateral intraovarian platelet-rich plasma injection and ovarian mechanical damage during injection on the functional ovarian reserve in rats for which a premature ovarian insufficiency model was created
Reproductive Biology, cilt.26, sa.4, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 26 Sayı: 4
- Basım Tarihi: 2026
- Doi Numarası: 10.1016/j.repbio.2026.101245
- Dergi Adı: Reproductive Biology
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, EMBASE, MEDLINE
- Anahtar Kelimeler: Ovarian mechanical damage, Platelet-rich plasma, Premature ovarian insufficiency, Rat model
- Dokuz Eylül Üniversitesi Adresli: Evet
Özet
We investigated the roles of bilateral intraovarian platelet-rich plasma (PRP) injection and ovarian mechanical damage during injection on the remaining functional ovarian reserve in rats with a premature ovarian insufficiency (POI) model. We analyzed serum anti-Mullerian hormone (AMH) levels, follicle-stimulating hormone (FSH) levels, follicle counts, fibrosis scores, histologic and immunohistochemical examinations, and related parameters. Twenty-eight adult female Wistar albino rats were randomly divided into four groups and treated with various combinations of cyclophosphamide, PRP, mechanical ovarian damage (MOD) and sodium chloride. In week eight, the rats were anesthetized, and their ovaries and blood samples were removed for examination. Although decreases in the numbers of primordial, secondary, and atretic follicles and increases in the numbers of primary and Graafian follicles were observed in the POI + MOD group compared with the POI + PRP + MOD group, these differences were not statistically significant. Serum FSH and AMH levels increased. In fibrosis scoring with Masson’s trichrome staining, the H score also increased. Immunohistochemical staining with Ki-67 showed less proliferation, immunohistochemical staining with Caspase-3 showed more apoptosis but these were not statistically significant. This is an experimental animal investigation with a comparatively modest sample size and the first study, to our knowledge, to compare the effects of intraovarian PRP injection with those of mechanical ovarian injury occurring during PRP administration. Although the study attempted to discuss statistically significant and insignificant differences as much as possible, more studies and studies with larger samples are needed in this area.