A fluorescent biosensor based on acetylcholine sterase and 5-oxazolone derivative immobilized in polyvinylchloride (PVC) matrix

Ozturk G., ALP S., Timur S.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, vol.47, no.3-4, pp.111-116, 2007 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 47 Issue: 3-4
  • Publication Date: 2007
  • Doi Number: 10.1016/j.molcatb.2007.03.009
  • Journal Name: JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED)
  • Page Numbers: pp.111-116
  • Keywords: acetylcholine, donepezil, 5-oxazolone, fluorescence emission, polymer film, ORGANOPHOSPHORUS INSECTICIDES, CHOLINESTERASE-INHIBITORS, DONEPEZIL HYDROCHLORIDE, AMPEROMETRIC BIOSENSOR, CARBAMATE PESTICIDES, FIBEROPTIC BIOSENSOR, ALZHEIMERS-DISEASE, ELECTRODES, COLUMN, FILMS
  • Dokuz Eylül University Affiliated: Yes

Abstract

A new 2-phenyl-4-[4-(1,4,7,10-tetraoxa-13-azacyclopentadecyl)benzylidene]-5-oxazolone (CPO) derivative was utilized to develop an optical acetylcholinesterase (AChE) biosensor in which the azlactone derivative was embedded in plasticized polyvinylchloride (PVC) matrix. The sensor system was calibrated for the detection of acetylcholine (ACh) and donepezil which is a competitive cholinesterase (ChE) inhibitor. Two different biosensing systems were developed by using AChE enzyme in solution and immobilized together with the fluorescent derivative (CPO) doped in transparent PVC membrane. The enzymatic hydrolysis of ACh was monitored by following changes in the pH induced fluorescence intensity. When AChE enzyme was immobilized in PVC matrix together with CPO, the sensitivity of the measuring system has increased approximately three times for ACh, in comparison to the sensing system where AChE enzyme was in solution phase.