Mastl is required for timely activation of APC/C in meiosis I and Cdk1 reactivation in meiosis II

Adhikari D., DİRİL M. K., Busayavalasa K., Risal S., Nakagawa S., Lindkvist R., ...Daha Fazla

JOURNAL OF CELL BIOLOGY, cilt.206, sa.7, ss.843-853, 2014 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 206 Sayı: 7
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1083/jcb.201406033
  • Dergi Adı: JOURNAL OF CELL BIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.843-853
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

In mitosis, the Greatwall kinase (called microtubule-associated serine/threonine kinase like [Mastl] in mammals) is essential for prometaphase entry or progression by suppressing protein phosphatase 2A (PP2A) activity. PP2A suppression in turn leads to high levels of Cdk1 substrate phosphorylation. We have used a mouse model with an oocyte-specific deletion of Mastl to show that Mastl-null oocytes resume meiosis I and reach metaphase I normally but that the onset and completion of anaphase I are delayed. Moreover, after the completion of meiosis I, Mastl-null oocytes failed to enter meiosis II (MII) because they reassembled a nuclear structure containing decondensed chromatin. Our results show that Mastl is required for the timely activation of anaphase-promoting complex/cyclosome to allow meiosis I exit and for the rapid rise of Cdk1 activity that is needed for the entry into MII in mouse oocytes.