The Use of MALDI-TOF Mass Spectrometry Technology in Molecular Analysis of Microbial Pathogenesis

Mehramouz B., Khodadadi E., Khodadadi E., Taghizadeh S., Ghanbarov K., KÖSE Ş., ...More

Biointerface Research in Applied Chemistry, vol.13, no.5, 2023 (Scopus) identifier

  • Publication Type: Article / Review
  • Volume: 13 Issue: 5
  • Publication Date: 2023
  • Doi Number: 10.33263/briac135.450
  • Journal Name: Biointerface Research in Applied Chemistry
  • Journal Indexes: Scopus
  • Keywords: infection, MALDI-TOF MS, mass spectrometry, microbiology, microorganism
  • Dokuz Eylül University Affiliated: Yes


Mass spectrometry is a method of evaluation used to identify which particles compose an example based on the ions' mass spectrum. Mass spectrometers can perform conventional detection and quantitation of target analytes. However, they can also be used for the rapid discovery of bacteria within a medical setting. Matrix-assisted laser desorption/ionization-time of flight mass spectrometer is one of the most prominent MS tools applied in biology, with its robust and accurate recognition of categories and types of a wide variety of Gram-positive and negative microorganisms. Mass spectrometry detection is based on determining a particular range of each kind and matching it with a comprehensive data source within the tool. Today's study describes the history and sample preparation of the MALDI-TOF MS technique. Moreover, the applications of MALDI-TOF MS microbial recognition in the center and the presence of antimicrobial resistance will be presented. Besides, the present restrictions and future use of MALDI-TOF MS in forthcoming daily scientific practice are reviewed. In this review, microorganisms will also be addressed in future clinical applications, primarily using MALDI-TOF MS in microbiology to identify and analyze antibiotic resistance.