EVALUATION OF THREE DIFFERENT CALCIUM SILICATE-BASED REPAIR MATERIALS IN TERMS OF MICROLEAKAGE: IN VITRO STUDY

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Çelikbay E., Kılıç Y., Tulgar M. M.

ESE Autumn Meeting 2026, Vilniaus, Lithuania, 23 - 24 October 2026, pp.1, (Summary Text)

  • Publication Type: Conference Paper / Summary Text
  • City: Vilniaus
  • Country: Lithuania
  • Page Numbers: pp.1
  • Dokuz Eylül University Affiliated: Yes

Abstract

EVALUATION OF THREE DIFFERENT CALCIUM SILICATE-BASED REPAIR MATERIALS IN TERMS OF MICROLEAKAGE: IN VITRO STUDY

Ece Çelikbay, Yağmur Kılıç, Mustafa Mert Tulgar

Aim

To evaluate and compare the sealing ability of three distinct calcium silicate-based repair materials—manually mixed Mineral Trioxide Aggregate (MTA Angelus) and two premixed bioceramic alternatives (Bio-C Repair and TotalFill BC RRM Fast Set Putty)—in simulated furcation perforation repair using a spectrophotometric microleakage dye penetration analysis.

 

Methodology

Sixty standardized dentin discs (2.5 mm thickness, 1.6 mm perforation diameter) obtained from extracted human maxillary molars were randomly distributed into three experimental groups (n=20 per group): Group 1 (MTA Angelus), Group 2 (Bio-C Repair), and Group 3 (TotalFill BC RRM Fast Set Putty). Following precise material placement and condensation according to standardized protocols using paper points and hand pluggers, specimens were incubated at 37°C with 100% humidity for 24 hours to allow initial polymerization. Microleakage was evaluated using a 2% methylene blue dye penetration test for 48 hours. Subsequently, the specimens were fully dissolved in 65% nitric acid for 48 hours. Quantitative chemical evaluation of dye microleakage was performed by measuring optical absorbance density at 550 nm using a UV/Vis spectrophotometer against a reference blank. Normal data distribution was verified using the Shapiro-Wilk test, and statistical variations among experimental groups were analyzed using one-way ANOVA followed by Tukey’s post-hoc pairwise comparison tests at a significance level of p<0.05.

 

Results

One-way ANOVA demonstrated statistically significant differences in microleakage values across all evaluated repair material groups (p<0.001). Manually mixed MTA Angelus demonstrated the lowest mean optical absorbance density values (0.255±0.100), indicating a statistically superior sealing ability and interfacial adaptation compared to the ready-to-use materials. Among the premixed formulations, TotalFill BC RRM Fast Set Putty exhibited a significantly lower mean microleakage absorbance (0.543±0.181) compared to Bio-C Repair (0.725±0.209), which was confirmed as a statistically significant difference by Tukey’s post-hoc analysis (p=0.003).

 

Conclusions

Within the limitations of this in vitro laboratory study, manually mixed MTA Angelus significantly outperformed the premixed bioceramic alternatives in terms of sealing performance and resistance to microleakage. The accelerated chemical setting mechanism and optimized handling characteristics of TotalFill BC RRM Fast Set Putty may offer superior early stabilization and interfacial adaptation compared to Bio-C Repair during the initial setting phase. This study received no external funding or financial support.