Poly(vinyl alcohol)/polyethyleneimine (PVA/PEI) blended monolithic cryogel columns for the depletion of haemoglobin from human blood

GÖKTÜRK BAŞAL I., Derazshamshir A., YILMAZ F., DENİZLİ A.

SEPARATION SCIENCE AND TECHNOLOGY, vol.51, no.11, pp.1787-1797, 2016 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 51 Issue: 11
  • Publication Date: 2016
  • Doi Number: 10.1080/01496395.2016.1183677
  • Journal Name: SEPARATION SCIENCE AND TECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1787-1797
  • Keywords: Cryogel, haemoglobin, IMAC, PEI-Cu2+, PVA, AFFINITY, RECOGNITION, SEPARATION, PROTEINS, DELIVERY, ALCOHOL), CAPTURE, POLYMER, BINDING, PEI
  • Dokuz Eylül University Affiliated: No

Abstract

We have synthesized PVA/PEI monolithic cryogel columns chelated with Cu2+ ions as a model adsorbent, which is capable of binding haemoglobin (Hb) from human blood. The goal of this study is to perform the depletion of Hb via a single and easy process to be useful in proteomic studies. PVA/PEI-Cu2+ cryogel columns were subjected to adsorption studies of Hb from both aqueous solution and human plasma to evaluate the extent of interaction between cryogel columns and Hb. The effects of experimental parameters, such as pH, Hb equilibrium concentration, adsorption time, temperature, and ionic strength, on Hb adsorption capacity were investigated.