Recommendations for the introduction of metagenomic next-generation sequencing in clinical virology, part II: bioinformatic analysis and reporting.

de Vries, Jutte; Brown, Julianne; Couto, Natacha; Beer, Martin; Le Mercier, Philippe; Sidorov, Igor; Papa, Anna; Fischer, Nicole; Munnink, Bas; Rodriquez, Christophe; Zaheri, Maryam; Sayiner, AYÇA; Hoenemann, Mario; Cataluna, Alba; Carbo, Ellen; Bachofen, Claudia; Kubacki, Jakub; Schmitz, Dennis; Tsioka, Katerina; Matamoros, Sebastien; Hoeper, Dirk; Hernandez, Marta; Puchhammer-Stoeckl, Elisabeth; Lebrand, Aitana; Huber, Michael; Simmonds, Peter; Claas, Eric; Lopez-Labrador, F.

doi:10.1016/j.jcv.2021.104812

Recommendations for the introduction of metagenomic next-generation sequencing in clinical virology, part II: bioinformatic analysis and reporting.

de Vries J. J. C., Brown J. R., Couto N., Beer M., Le Mercier P., Sidorov I., ...Daha Fazla

Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, cilt.138, ss.104812, 2021 (SCI-Expanded, Scopus)

Yayın Türü: Makale / Tam Makale
Cilt numarası: 138
Basım Tarihi: 2021
Doi Numarası: 10.1016/j.jcv.2021.104812
Dergi Adı: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, CAB Abstracts, EMBASE, MEDLINE, Public Affairs Index, Veterinary Science Database
Sayfa Sayıları: ss.104812
Anahtar Kelimeler: Viral metagenomics, NGS, HTS, Bioinformatics, Pipeline, Diagnostics, Pathogens
Dokuz Eylül Üniversitesi Adresli: Evet

Özet

Metagenomic next-generation sequencing (mNGS) is an untargeted technique for determination of microbial DNA/RNA sequences in a variety of sample types from patients with infectious syndromes. mNGS is still in its early stages of broader translation into clinical applications. To further support the development, implementation, optimization and standardization of mNGS procedures for virus diagnostics, the European Society for Clinical Virology (ESCV) Network on Next-Generation Sequencing (ENNGS) has been established. The aim of ENNGS is to bring together professionals involved in mNGS for viral diagnostics to share methodologies and experiences, and to develop application guidelines. Following the ENNGS publication Recommendations for the introduction of mNGS in clinical virology, part I: wet lab procedure in this journal, the current manuscript aims to provide practical recommendations for the bioinformatic analysis of mNGS data and reporting of results to clinicians.