Genome-wide analysis of endogenously expressed ZEB2 binding sites reveals inverse correlations between ZEB2 and GalNAc-transferase GALNT3 in human tumors

Balcik-Ercin, PELİN; Cetin, Metin; Yalim-Camci, Irem; Odabas, Gorkem; Tokay, Nurettin; Sayan, A.; Yagci, Tamer

doi:10.1007/s13402-018-0375-7

Genome-wide analysis of endogenously expressed ZEB2 binding sites reveals inverse correlations between ZEB2 and GalNAc-transferase GALNT3 in human tumors

Balcik-Ercin P., Cetin M., Yalim-Camci I., Odabas G., Tokay N., Sayan A. E., ...Daha Fazla

CELLULAR ONCOLOGY, cilt.41, sa.4, ss.379-393, 2018 (SCI-Expanded, Scopus)

Yayın Türü: Makale / Tam Makale
Cilt numarası: 41 Sayı: 4
Basım Tarihi: 2018
Doi Numarası: 10.1007/s13402-018-0375-7
Dergi Adı: CELLULAR ONCOLOGY
Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
Sayfa Sayıları: ss.379-393
Anahtar Kelimeler: ZEB2, GALNT3, Antibody validation, ChIP-sequencing, Gene regulation, Tissue expression
Dokuz Eylül Üniversitesi Adresli: Hayır

Özet

ZEB2 is a transcriptional repressor that regulates epithelial-to-mesenchymal transition (EMT) through binding to bipartite E-box motifs in gene regulatory regions. Despite the abundant presence of E-boxes within the human genome and the multiplicity of pathophysiological processes regulated during ZEB2-induced EMT, only a small fraction of ZEB2 targets has been identified so far. Hence, we explored genome-wide ZEB2 binding by chromatin immunoprecipitation-sequencing (ChIP-seq) under endogenous ZEB2 expression conditions.