Genome-wide analysis of endogenously expressed ZEB2 binding sites reveals inverse correlations between ZEB2 and GalNAc-transferase GALNT3 in human tumors

Balcik-Ercin P., Cetin M., Yalim-Camci I., Odabas G., Tokay N., Sayan A. E., ...Daha Fazla

CELLULAR ONCOLOGY, cilt.41, sa.4, ss.379-393, 2018 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 41 Sayı: 4
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1007/s13402-018-0375-7
  • Dergi Adı: CELLULAR ONCOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.379-393
  • Anahtar Kelimeler: ZEB2, GALNT3, Antibody validation, ChIP-sequencing, Gene regulation, Tissue expression, EPITHELIAL-MESENCHYMAL TRANSITION, SMAD-INTERACTING PROTEIN-1, N-ACETYLGALACTOSAMINYLTRANSFERASE 3, ALPHA-D-GALACTOSAMINE, CANCER-CELLS, E-CADHERIN, MIR-200 FAMILY, CISPLATIN RESISTANCE, O-GLYCOSYLATION, STEM-CELLS
  • Dokuz Eylül Üniversitesi Adresli: Hayır

Özet

ZEB2 is a transcriptional repressor that regulates epithelial-to-mesenchymal transition (EMT) through binding to bipartite E-box motifs in gene regulatory regions. Despite the abundant presence of E-boxes within the human genome and the multiplicity of pathophysiological processes regulated during ZEB2-induced EMT, only a small fraction of ZEB2 targets has been identified so far. Hence, we explored genome-wide ZEB2 binding by chromatin immunoprecipitation-sequencing (ChIP-seq) under endogenous ZEB2 expression conditions.