Resveratrol Reduces Matrix Metalloproteinase-2 Activity Induced by Oxygen-Glucose Deprivation and Reoxygenation in Human Cerebral Microvascular Endothelial Cells

ÇAVDAR Z., YÜKSEL EĞRİLMEZ M., ALTUN Z. S., ARSLAN N., YENER N., SAYIN O., ...Daha Fazla

INTERNATIONAL JOURNAL FOR VITAMIN AND NUTRITION RESEARCH, cilt.82, sa.4, ss.267-274, 2012 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 82 Sayı: 4
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1024/0300-9831/a000119
  • Dergi Adı: INTERNATIONAL JOURNAL FOR VITAMIN AND NUTRITION RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.267-274
  • Anahtar Kelimeler: cerebral endothelial cell, oxygen-glucose deprivation, resveratrol, MMP-2, ROS, OXIDATIVE STRESS, ISCHEMIA-REPERFUSION, PROTEIN ASSAY, BRAIN-DAMAGE, INJURY, INVOLVEMENT, DISRUPTION, EXPRESSION, STROKE, SYSTEM
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

The main pathophysiology in cerebral ischemia is the structural alteration in the neurovascular unit, coinciding with neurovascular matrix degradation. Among the human matrix metalloproteinases (MMPs), MMP-2 and -9, known as gelatinases, are the key enzymes for degrading type IV collagen, which is the major component of the basal membrane that surrounds the cerebral blood vessel. In the present study, we investigated the effects of resveratrol on cytotoxicity, reactive oxygen species (ROS), and gelatinases (MMP-2 and -9) in human cerebral microvascular endothelial cells exposed to 6 hours of oxygen-glucose deprivation and a subsequent 24 hours of reoxygenation with glucose (OGD/R), to mimic ischemia/reperfusion in vivo. Lactate dehydrogenase increased significantly, in comparison to that in the normoxia group. ROS was markedly increased in the OGD/R group, compared to normoxia. Correspondingly, ROS was significantly reduced with 50 p,M of resveratrol. The proMMP-2 activity in the OGD/R group showed a statistically significant increase from the control cells. Resveratrol preconditioning decreased significantly the proMMP-2 in the cells exposed to OGD/R in comparison to that in the OGD/R group. Our results indicate that resveratrol regulates MMP-2 activity induced by OGD/R via its antioxidant effect, implying a possible mechanism related to the neuroprotective effect of resveratrol.