Immunohistochemical detection of PS2 protein and heat shock protein-70 in pancreatic adenocarcinomas. relationship with disease extent and patient survival

Sagol Ö., Tuna B., Coker A., Karademir S., BARLIK OBUZ F., Astarcioglu H., ...More

PATHOLOGY RESEARCH AND PRACTICE, vol.198, no.2, pp.77-84, 2002 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 198 Issue: 2
  • Publication Date: 2002
  • Doi Number: 10.1078/0344-0338-00190
  • Journal Name: PATHOLOGY RESEARCH AND PRACTICE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.77-84
  • Keywords: HSP-70, pS2, pancreatic adenocarcinoma, HEAT-SHOCK PROTEINS, PROGNOSTIC-FACTORS, DIFFERENTIAL EXPRESSION, BREAST-CANCER, ESTROGEN-RECEPTOR, P53 EXPRESSION, CARCINOMA, ASSOCIATION, HSP90, HSP70
  • Dokuz Eylül University Affiliated: Yes

Abstract

We investigated pS2 and HSP-70 protein expression in 36 pancreatic adenocarcinomas for their effect on disease extent and patient outcome. The cases were reviewed, histologically diagnosed, typed, graded, and staged. Lymphatic vessel, blood vessel and perineural invasion as well as lymph node, resection margin and adjacent organ involvements were re-evaluated. The standard streptavidin biotin immunperoxidase method was used for immunostaining with pS2 and HSP-70 antibodies. Cytoplasmic staining with both antibodies was scored semiquantitatively. The scores were compared with histopathological prognostic parameters using statistical methods. Standard prognostic parameters and staining scores were tested by survival analysis in terms of their effect on survival.