Separation of D,L-Ampicillin by ligand exchange-micellar electrokinetic chromatography

Sari D., Derazshamshir A., Asir S., Göktürk Başal I., Yılmaz F., Denizli A.

BIOINTERFACE RESEARCH IN APPLIED CHEMISTRY, cilt.9, sa.6, ss.4522-4533, 2019 (ESCI) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 9 Sayı: 6
  • Basım Tarihi: 2019
  • Doi Numarası: 10.33263/briac96.522533
  • Dergi Adı: BIOINTERFACE RESEARCH IN APPLIED CHEMISTRY
  • Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), Scopus
  • Sayfa Sayıları: ss.4522-4533
  • Anahtar Kelimeler: Ampicillin, Capillary Electrophoresis, Micelle, CAPILLARY-ELECTROPHORESIS, CHIRAL SEPARATION, AMINO-ACIDS, IONIC LIQUIDS, ORGANIC-SOLVENTS, ENANTIOSEPARATION, DRUGS, ANTIBIOTICS, COMPLEX, STATIONARY
  • Dokuz Eylül Üniversitesi Adresli: Hayır

Özet

In this study, D,L-ampicillin separation was carried out by ligand exchange-micellar electrokinetic chromatography method using L-Lysine monohydrochloride as a ligand and copper (II) sulfate pentahydrate is a central ion supplier. Isomeric separations were performed using capillary electrophoresis (CE) instrument, in which SDS-L-Lys-Cu+2 micelle complex was used as a pseudostationary phase. The effect of pH, SDS amount, applied electrical field, pressure, organic solvent ratio and ampicillin D,L-ratios were investigated. Fast,inexpensive and sensitive approach for the simultaneous separation of D,L-ampicillin in both aqueous and real antibiotic sample was performed using CE coupled with UV detector. The separation was achieved in a short period of 7 minutes with high-sensitivity andlow-detection limit of 1.25 mu M by the developed SDS-L-Lys-Cu+2 micelle-chiral selector complexes without using any extra process such as imprinting or spacer arms for the immobilization of the ligands.