Recruitment of solid lipid nanoparticles for the delivery of CRISPR/Cas9: primary evaluation of anticancer gene editing

AKBABA H., EREL AKBABA G., ŞENTÜRK Ş.

NANOMEDICINE, cilt.16, sa.12, ss.963-978, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 16 Sayı: 12
  • Basım Tarihi: 2021
  • Doi Numarası: 10.2217/nnm-2020-0412
  • Dergi Adı: NANOMEDICINE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, PASCAL, BIOSIS, Biotechnology Research Abstracts, Chemical Abstracts Core, EMBASE, MEDLINE
  • Sayfa Sayıları: ss.963-978
  • Anahtar Kelimeler: 3D ternary plot, CRISPR, Cas9, gene delivery, PX458, solid lipid nanoparticle, transfection, TRANSFECTION EFFICIENCY, IN-VITRO, CLINICAL DEVELOPMENT, CELL TRANSFECTION, CATIONIC LIPIDS, RECENT PROGRESS, VIVO, VECTORS, STABILITY, VEHICLES
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

Aim: The CRISPR/Cas9 system is a promising gene-editing tool for various anticancer therapies; however, development of a biocompatible, nonviral and efficient delivery of CRISPR/Cas9 expression systems remains a challenge. Materials & methods: Solid lipid nanoparticles (SLNs) were produced based on pseudo and 3D ternary plots. Obtained SLNs and their complexes with PX458 plasmid DNA were characterized and evaluated in terms of cytotoxicity and transfection efficiency. Results: SLNs were found to be nanosized, monodispersed, stable and nontoxic. Furthermore, they revealed similar transfection efficiency as the positive control. Conclusion: Overall, we have achieved a good SLN basis for CRISPR/Cas9 delivery and have the potential to produce SLNs with targeted anticancer properties by modifying production parameters and components to facilitate translating CRISPR/Cas9 into preclinical studies.