Saturation of the secretory pathway by overexpression of a hookworm (Necator americanus) protein (Na-ASP1)
Methods in Molecular Biology, cilt.389, ss.65-75, 2007 (Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 389
- Basım Tarihi: 2007
- Doi Numarası: 10.1385/1-59745-456-7:65
- Dergi Adı: Methods in Molecular Biology
- Derginin Tarandığı İndeksler: Scopus
- Sayfa Sayıları: ss.65-75
- Anahtar Kelimeler: Heterologous protein secretion, Necator americanus secretory protein (Na-ASP1), Pichia pastoris
- Dokuz Eylül Üniversitesi Adresli: Hayır
Özet
Human hookworm infection is one of the most significant parasitic infections, and a leading global cause of anemia and malnutrition of adults and children in rural areas of the tropics and subtropics. Necator americanus secretory protein (Na-ASP1), which is a potential vaccine candidate against hookworm infections, has been expressed in Pichia pastoris. Na-ASP1 protein was expressed extracellulary by employing the leader sequence of the a-mating factor of Saccharomyces cerevisiae. Most of the protein produced by single copy clones was secreted outside the cell. The Na-ASP1 steady state mRNA levels of the clones were correlated to their Na-ASP1 gene copy number. However, increasing gene copy number of Na-ASP1 protein in P. pastoris saturated secretory capacity and therefore, decreased the amount of secreted protein in clones harboring multiple copies of Na-ASP1 gene. © Humana Press Inc.,.