Saturation of the secretory pathway by overexpression of a hookworm (Necator americanus) protein (Na-ASP1)

İNAN M., Fanders S. A., Zhang W., Hotez P. J., Zhan B., Meagher M. M.

Methods in Molecular Biology, cilt.389, ss.65-75, 2007 (Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 389
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1385/1-59745-456-7:65
  • Dergi Adı: Methods in Molecular Biology
  • Derginin Tarandığı İndeksler: Scopus
  • Sayfa Sayıları: ss.65-75
  • Anahtar Kelimeler: Heterologous protein secretion, Necator americanus secretory protein (Na-ASP1), Pichia pastoris
  • Dokuz Eylül Üniversitesi Adresli: Hayır

Özet

Human hookworm infection is one of the most significant parasitic infections, and a leading global cause of anemia and malnutrition of adults and children in rural areas of the tropics and subtropics. Necator americanus secretory protein (Na-ASP1), which is a potential vaccine candidate against hookworm infections, has been expressed in Pichia pastoris. Na-ASP1 protein was expressed extracellulary by employing the leader sequence of the a-mating factor of Saccharomyces cerevisiae. Most of the protein produced by single copy clones was secreted outside the cell. The Na-ASP1 steady state mRNA levels of the clones were correlated to their Na-ASP1 gene copy number. However, increasing gene copy number of Na-ASP1 protein in P. pastoris saturated secretory capacity and therefore, decreased the amount of secreted protein in clones harboring multiple copies of Na-ASP1 gene. © Humana Press Inc.,.