The effects of cervical mucous glycodelin-A, granulocyte colony-stimulating factor and L-selectin on pregnancy outcomes in in-vitro fertilization-embryo transfer cycles


Güngör M., Doǧan E., POSACI C., UYSAL S., Saatli B., Güleki B.

Turkiye Klinikleri Jinekoloji Obstetrik, vol.22, no.4, pp.220-227, 2012 (Scopus) identifier

  • Publication Type: Article / Article
  • Volume: 22 Issue: 4
  • Publication Date: 2012
  • Journal Name: Turkiye Klinikleri Jinekoloji Obstetrik
  • Journal Indexes: Scopus, Academic Search Premier, CAB Abstracts, EMBASE, Veterinary Science Database
  • Page Numbers: pp.220-227
  • Keywords: Fertilization in vitro, Granulocyte colony-stimulating factor, L-selectin, PAEP protein, human
  • Dokuz Eylül University Affiliated: Yes

Abstract

Objective: To investigate the benefits of cervical mucous glycodelin-A, granulocyte colony-stimulating factor (G-CSF) and L-selectin levels as a marker of endometrial receptivity in in-vitro fertilization/embryo transfer cycles (IVF-ET). Material and Methods: In this prospective cohort study cervical mucus samples were collected during oocyte pick up and embryo transfer from 56 IVF-ET patients. Blood samples were collected during embryo transfer only. The glycodelin-A, G-CSF and L-selectin levels in cervical mucus and blood samples were measured and the results were compared with the pregnancy rates. Results: Cervical mucus G-CSF and L-Selectin levels measured at embryo transfer were significantly higher than those measured during oocyte pick-up (p=0.04 and p=0.002, respectively). Mean cervical mucous G-CSF levels were significantly higher in pregnant patients compared to non pregnant patients during embryo transfer (p=0.024). When 1365 pg/ml was set as cut-off point for G-CSF levels in predicting pregnancy, sensitivity and specificity were 76% and 61.3%, respectively. However, there were no significant relationship between the glycodelin-A and L-selectin levels in the cervical mucus and pregnancy outcomes. Conclusion: Investigating markers of endometrial receptivity in the cervical mucus during embryo transfer is a novel and non-invasive method in IVF cycles. G-CSF may be an important marker in predicting the implantation. Copyright © 2012 by Türkiye Klinikleri.