Non-repressing carbon sources for alcohol oxidase (AOX1) promoter of Pichia pastoris
Journal of Bioscience and Bioengineering, cilt.92, sa.6, ss.585-589, 2001 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 92 Sayı: 6
- Basım Tarihi: 2001
- Doi Numarası: 10.1016/s1389-1723(01)80321-2
- Dergi Adı: Journal of Bioscience and Bioengineering
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.585-589
- Anahtar Kelimeler: AOX1 promoter, Carbon catabolite repression, Pichia pastoris
- Dokuz Eylül Üniversitesi Adresli: Hayır
Özet
The growth of Pichia pastoris in a mixture of either glycerol or glucose and methanol follows a diauxic growth, with C1 utilizing enzymes being repressed. Therefore, these carbon sources can not be used as a mixture with methanol to simultaneously grow P. pastoris and induce C1 utilizing enzymes, especially in a shake flask cultures of AOX-deficient P. pastoris. Among the alternative carbon sources tested, alanine, sorbitol, mannitol and trehalose, did not repress β-gal production when methanol was used as an inducer in mut- strain of P. pastoris. Our results show that either one of alanine, sorbitol, mannitol or trehalose can be used as a sole carbon and energy source for P. pastoris, although the doubling time on trehalose was very long. Mut- strains growing in media containing trehalose, alanine, sorbitol and mannitol with methanol (0.5%) as an inducing agent expressed as much or higher amount of β-gal as compared to the mut+ growing in methanol containing media.