Atıf İçin Kopyala
Schäfer‐Somi S., Beceriklisoy H., Walter I., Sabitzer S., Klein D., Kanca H., ...Daha Fazla
REPRODUCTION IN DOMESTIC ANIMALS, cilt.44, sa.s2, ss.103-108, 2009 (SCI-Expanded)
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Yayın Türü:
Makale / Tam Makale
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Cilt numarası:
44
Sayı:
s2
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Basım Tarihi:
2009
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Doi Numarası:
10.1111/j.1439-0531.2009.01444.x
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Dergi Adı:
REPRODUCTION IN DOMESTIC ANIMALS
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Derginin Tarandığı İndeksler:
Science Citation Index Expanded (SCI-EXPANDED), Scopus
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Sayfa Sayıları:
ss.103-108
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Dokuz Eylül Üniversitesi Adresli:
Hayır
Özet
ContentsThe aim of the study was to investigate the expression of major histocompatibility complex (MHC)‐I and ‐II in uterine tissues from pregnant and non‐pregnant bitches, taken at different time periods after mating. The pregnant bitches were ovariohysterectomized during the pre‐implantation (group 1, n = 4), implantation (group 2, n = 7) and placentation stage (group 3, n = 7). Non‐pregnant animals in diestrus served as controls (group 4, n = 7). The expression of MHC‐ I and ‐II in salpinx, apex, middle horn, corpus uteri and at implantation sites was investigated by immunohistochemistry as well as qualitative and quantitative RT‐PCR; MHC‐I mRNA was detected in all tissues and with quantitative RT‐PCR, and no significant changes were detected until placentation. Immunohistologically, at the apex and corpus site, the average number of MHC‐II positive cells increased from the pre‐implantation to the post‐implantation stage (apex: 1.54 ± 1.21 to 3.82 ± 2.93; corpus: 1.62 ± 1.9 to 5.04 ± 4.95; p < 0.05). The greatest numbers of MHC‐II positive cells were observed at placentation sites (6.64 ± 5.9). In parallel, a marked increase in the relative mRNA expression of MHC‐II in uterine tissues was assessed from the pre‐implantation to the placentation stage (relative to Glycerinaldehyd‐3‐phosphate‐Dehydrogenase (GAPDH): 6.9 ± 9.5, 8.4 ± 5.8, p > 0.05). Immunohistologically, in the salpinx, significantly greater numbers of MHC‐II positive cells were found in the tissues of pregnant animals than in the control group (p < 0.05). It is proposed that the increase in MHC‐II is pregnancy‐related, even though the impact on maintenance of canine pregnancy is still unclear.