Akademik Veri Yönetim Sistemi
TBS International Biochemistry Congress 2022, 33rd National Biochemistry Congress, İzmir, Türkiye, 26 - 30 Ekim 2022, cilt.47, sa.2, ss.64-65
BACKGROUND AND AIM: The genus Thymus, belonging to the
family Lamiaceae, has multiple biological and pharmacological
properties, including antimicrobial, antiseptic, antiviral and antifungal activities. This study aimed to determine the cytotoxic effect
and metastatic potential of methanol/water phase extract prepared
from Thymus vulgaris (TV) in human breast adenocarcinoma cell
line (BT-474) by combining it with the chemotherapeutic agent
paclitaxel (PAC).
MATERIALS and METHODS: The IC25 and IC50 concentrations
of TV and PAC in BT-474 cells were determined by the modified
MTT method we developed in our laboratory.Cell migration as
an indicator of metastatic potential; with in vitro wound healing
method, colony formation potential was evaluated with Spheroid
Model. The evaluation of the data was made with SPSS program.
Conformity of the variables to the normal distribution ANOVA was
used to compare the groups.
RESULTS: In wound healing assays, TV50 dose was found to be
statistically significant in “cell migration rate” measurements compared with treatment groups.P<0,05. When the spheroidal formation in the 10-day 3D agarose gel is taken into consideration, there is
no significant statistical difference between the PAC 50 and TS50
groups, while in the combined group; spheroid formations larger
than the reference value were statistically significantly lower. A
significant reduction in spheroidal formation was observed in combined (TV25+PAC25) and TV50 doses compared to the control
group. P<0.05
CONCLUSION: These findings are due to the fact that TV therapy
in breast adenocarcinoma cells; especially when combined with
PAC, it reveals that it may be effective in preventing tumor cells
from becoming spheroidal and tumor cell migration.
Keywords: metastasis, migration, invasion, thymus vulgaris