Akademik Veri Yönetim Sistemi
Comparative Clinical Pathology, cilt.34, sa.4, ss.567-574, 2025 (Scopus)
Background: Swiss Albino strain mice are commonly used laboratory animals in research and veterinary science studies. Reference intervals can be helpful for evaluating clinical blood results. Especially for this strain, there is limited data in the literature regarding reference intervals calculated according to the IFCC EP28-A3C guideline. Purpose: The aim was to determinethe reference intervals and standard deviations of various analytes in Swiss Albino strain mice sera. These analytes include alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), γ-glutamyl transferase (GGT), lactate dehydrogenase (LDH), total bilirubin (T-Bil), total protein (TP), albumin, total cholesterol (TC), triglyceride, glucose, creatinine, urea, uric acid, calcium, magnesium, phosphorus, sodium, potassium, and chloride. Methods: For the study, blood samples were collected from adult (12–24 weeks old) and healthy Swiss Albino mice (134 males and 129 females) obtained from various laboratory animal breeding centers in Turkey, and they were analyzed using the Mindray BS-240 Vet brand wet chemistry automated analyzer. The calculations were performed both for the sexes (male + female) and separately for each sex. Reference intervals were calculated using non-parametric methods (median and 2.5th and 97.5th percentiles). Results: The study established reference intervals for 19 serum biochemical analytes in Swiss Albino mice, both for the combined sexes (male + female) and separately for each sex. Significant sex-related differences were identified for several analytes, including higher AST, LDH, TP, albumin, and calcium levels in females, whereas males exhibited higher TC, glucose, phosphorus, and urea levels (p range: 0.05–0.001). Conclusion: The reference intervals of serum biochemical analytes determined in this study will serve as a guide for clinical pathology diagnosis in future studies conducted with Swiss Albino strain mice.