Masonry infill walls are unavoidable parts of any building to create a separation between internal space and external environment. In general, there are some prevalent openings in the infill wall due to functional needs, architectural considerations or aesthetic concerns. In current design practice, the strength and stiffness contribution of infill walls is not considered. However, the presence of infill walls may decisively influence the seismic response of structures subjected to earthquake loads and cause a different behavior from that predicted for a bare frame. Furthermore, partial openings in the masonry infill wall are significant parameter affecting the seismic behavior of infilled frames thereby decreasing the lateral stiffness and strength. The possible effects of openings in the infill wall on seismic behavior of RC frames is analytically studied by means of pushover analysis of several bare, partially and fully infilled frames having different bay and story numbers. The stiffness loss due to partial opening is introduced by the stiffness reduction factors which are developed from finite element analysis of frames considering frame-infill interaction. Pushover curves of frames are plotted and the maximum base shear forces, the yield displacement, the yield base shear force coefficient, the displacement demand, interstory drift ratios and the distribution of story shear forces are determined. The comparison of parameters both in terms of seismic demand and capacity indicates that partial openings decisively influences the nonlinear behavior of RC frames and cause a different behavior from that predicted for a bare frame or fully infilled frame.