10.International Academic Studies Conference, Elazığ, Turkey, 28 - 30 November 2022, pp.159-168
Dental plaque bacteria are responsible for the development of periodontal disease in dogs. The aim of this study was to determine the presence of pathogenic bacteria associated with periodontal disease in the dental plaques of spayed Pitbull dogs. Plaque and subgingival swap samples were obtained from 27 male and 31 female dogs. Samples were analyzed with PCR for Porphyromonas gingivalis, Porphyromonas gulae, Treponema denticola, Tannerella forsythia, Capnocytophaga ochracea, Capnocytophaga sputigena, Prevotella intermedia, Prevotella nigrescens, Campylobacter rectus, Aggregatibacter actinomycetemcomitans, and Eikenella corrodens. Positive samples (52/58) with 16S rRNA PCR were determined as 89.65% and 6 samples were negative. All samples were confirmed except P.nigrescens and A.actinomycetemcomitans. P.gingivalis (50/52) was detected in 96.15% and P.gulae and P.intermedia (33/52) were in 63.46%. 1.9% of T. denticola and C. rectus (1/52) were confirmed only in male dogs. Also, gender did not affect the presence of pathogenic bacteria. As a result of the research, bacterial variations strongly associated with Pitbull periodontal diseases were obtained.