The Analytical Performance of a Microplate Method for Total Sulfhydryl Measurement in Biological Samples

Taylan E., Resmi H.

TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI, vol.35, no.3, pp.275-278, 2010 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 35 Issue: 3
  • Publication Date: 2010
  • Journal Name: TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.275-278
  • Keywords: Oxidative stress, sulfhydryl groups, Ellman's reagent, microplate method
  • Dokuz Eylül University Affiliated: Yes

Abstract

Objective: Sulfhydryl groups of protein, glutathione and free cysteine are readily oxidized to disulfides by oxidants, and are important indicators of oxidative stress. Our aim was to apply the Ellman method for assay of sulfhydryl groups to microplate scale and to determine this method's analytical performance.