The Analytical Performance of a Microplate Method for Total Sulfhydryl Measurement in Biological Samples
TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI, cilt.35, sa.3, ss.275-278, 2010 (SCI-Expanded, Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 35 Sayı: 3
- Basım Tarihi: 2010
- Dergi Adı: TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.275-278
- Anahtar Kelimeler: Oxidative stress, sulfhydryl groups, Ellman's reagent, microplate method
- Dokuz Eylül Üniversitesi Adresli: Evet
Özet
Objective: Sulfhydryl groups of protein, glutathione and free cysteine are readily oxidized to disulfides by oxidants, and are important indicators of oxidative stress. Our aim was to apply the Ellman method for assay of sulfhydryl groups to microplate scale and to determine this method's analytical performance.