Flurbiprofen inhibits capsaicin induced calcitonin gene related peptide release from rat spinal cord via an endocannabinoid dependent mechanism

Seidel K., Hamza M., ATEŞ M., Guhring H.

NEUROSCIENCE LETTERS, cilt.338, sa.2, ss.99-102, 2003 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 338 Sayı: 2
  • Basım Tarihi: 2003
  • Doi Numarası: 10.1016/s0304-3940(02)01366-6
  • Dergi Adı: NEUROSCIENCE LETTERS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.99-102
  • Anahtar Kelimeler: spinal superperfusion, rat, calcitonin gene related peptide, flurbiprofen, delta-9 tetrahydrocannabinol, endocannabinoids, nociception, prostaglandin E-2, INFLAMMATION, RECEPTORS, INDUCTION
  • Dokuz Eylül Üniversitesi Adresli: Hayır

Özet

Calcitonin gene related peptide (CGRP) is involved in nociceptive transmission and modulation at the spinal level. In the spinal superperfusion model, Delta(9) tetrahydrocannabinol inhibited capsaicin induced CGRP release in a concentration dependent manner. Similarly, flurbiprofen (3 muM) inhibited spinal CGRP release. This inhibition was reversed by the CB1 antagonist AM-251 (1 muM), but not by co-administration of prostaglandin E-2 (PGE(2); 285 nM). AM-251 had no modulatory effect on flurbiprofen-induced cyclooxygenase (COX) inhibiting capacity as shown by PGE2 levels. Furthermore, the phospholipase A(2) inhibitor palmityl trifluromethyl ketone (15 muM) reversed flurbiprofen's inhibitory effect. In conclusion the present work provides evidence on the shift of arachidonic acid metabolism towards endocannabinoids formation in response to COX inhibition as a mechanism for flurbiprofen inhibitory effect on spinal CGRP release. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.