Effects of Apilarnil on Type 2 Diabetes-Induced IRS-1/PI3K/Akt Mediated Insulin Resistance in Male Rats

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Karagözoğlu F., Şahin E., Melek Ş., Bediz Şahin S., Koca R. H., Yüksel H., ...Daha Fazla

ACS OMEGA, cilt.10, sa.23, ss.25027-25038, 2025 (SCI-Expanded, Scopus)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 10 Sayı: 23
  • Basım Tarihi: 2025
  • Dergi Adı: ACS OMEGA
  • Derginin Tarandığı İndeksler: Scopus, Science Citation Index Expanded (SCI-EXPANDED), Chemical Abstracts Core, Directory of Open Access Journals
  • Sayfa Sayıları: ss.25027-25038
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

Diabetes is one of the most important chronic and metabolichealthproblemsseenasanepidemicof the21stcentury.The incidenceof diabetes is gradually increasing, anddifferentmethods are usedtotreat it.Apitherapyproducts suchashoney, propolis, royal jelly, andbeevenomcanbeusedasanalternativetotreatdiabetesandreduce symptoms.Oneof theseapitherapyproducts isApilarnil,whichhasnot yet become popular. The hypolipidemic, hepatoprotective, androgenic, anabolic, and immunesystemenhancingpropertiesofApilarnil indicate that itmaybeeffectiveinthetreatmentof type2diabetesor inreducing the symptoms.The studyaimed todetermine the therapeutic effect of Apilarniloninsulinresistanceandthepossibleunderlyingmechanism. In thestudy,408week-oldmaleWistaralbinoratswereused.Theratswere dividedinto5groupsasControl,Diabetes,Diabetes+Api1(125mg/kg/ daylyophilizedApilarnil),Diabetes+Api2(250mg/kg/daylyophilizedApilarnil)andDiabetes+Api3(500mg/kg/daylyophilized Apilarnil).Eachgroupconsistedof8rats.Apilarnildoseswereadministeredbyoralgavagewith1mLofdistilledwater,5daysina week, during the last6weeks.The levelsofNrf2,NF-κB,TNF-α, total IRS-1, p-IRS-1, PI3Kp85, totalAkt, p-Akt, IL-1B, and GLUT4proteinsweredeterminedintargettissuesbyWesternblotting.Alanineaminotransferase(ALT),aspartateaminotransferase (AST),high-densitylipoprotein(HDL), low-densitylipoprotein(LDL), total cholesterol (TC), andtriglyceride(TG)levelswere detectedintheserum. Serumsuperoxidedismutase(SOD), catalase(CAT), glutathioneperoxidase(GSH-Px),malondialdehyde (MDA),andinsulinlevelsweredeterminedbytheELISAmethod.Accordingtothedataobtainedinthestudy, theapplicationof Apilarnil, comparedtothediabetesgroup,balancedsomefindings,butdidnotdemonstratea fullyantidiabeticeffect.