Akademik Veri Yönetim Sistemi
9th International Congress of Molecular Medicine, İstanbul, Türkiye, 18 - 20 Aralık 2023, ss.26-27
Tissue scaffolds provide a structural
environment for cells to adhere and
proliferate. Thermoplastic polyurethanes
(TPUs) are linear polymers that have high
biocompatibility, biodegradability and superior
viscoelastic behaviour. They are used in
medical applications such as wound dressings.
3D printing is an innovative tool for tissue
engineering. Dermal fibroblasts play an
essential role in skin wound healing.
Polygonum cognatum Meissn. (PC) is a wild
plant rich in vitamin C and carotenoids. It has
antioxidant, antimicrobial, and antidiabetic
activities. The aim of this study is to investigate
the effect of PC on the viability, distribution
and collagen expression of human dermal
fibroblasts (HDFs) seeded on 3D TPU scaffolds.
3D TPU scaffolds were prepared using a fused
deposition modeling printer. HDFs were
seeded on 3D TPU scaffolds and treated with
250 μg/mL of PC for 24 and 48 h. Cell viability
was measured by WST-1 assay. Cell distribution
was analyzed by hematoxylin and eosin (H&E)
staining. Collagen expression in HDFs was
stained with Masson’s trichrome (MT). The
viability of HDFs on 3D TPU scaffolds treated
with PC was significantly higher than HDFs on
3D TPU scaffolds at 48 h. H&E staining
demonstrated that HDFs on 3D scaffolds
treated with PC maintained their tissue-specific
morphology and have larger cell and nucleus
sizes. Collagen expression by MT staining was
more intense in HDFs on 3D scaffolds treated
with PC. Our results show that PC increases
viability and collagen expression of HDFs on 3D TPU scaffolds and may promote wound healing
through modulation of cell behaviours.