INTERNATIONAL BIODETERIORATION & BIODEGRADATION, cilt.55, sa.1, ss.25-28, 2005 (SCI-Expanded)
Biological degradation of 2,4-dichlorophenol (DCP) by Pseudonionas putida CP1 was investigated in batch shake flask-cultures. Experiments were carried out at initial DCP concentrations between 50 and 750mg l(-1) and the rate and extent of DCP degradation were quantified. With increasing initial DCP concentrations in shake-flask cultures, percentage removal of DCP decreased and residual DCP concentrations increased proportionally. The rate of DCP degradation increased with increasing initial DCP concentration up to 577 mg DCPl(-l). Further increases in DCP concentration caused decreases in the rate of degradation because of substrate inhibition. A kinetic model based on substrate inhibition was proposed. Rate and inhibition constants were determined using the experimental data. (C) 2004 Elsevier Ltd. All rights reserved.