Kinetics of 2,4-dichlorophenol degradation by Pseudomonas putida CP1 in batch culture

Kargi F., EKER S.

INTERNATIONAL BIODETERIORATION & BIODEGRADATION, vol.55, no.1, pp.25-28, 2005 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 55 Issue: 1
  • Publication Date: 2005
  • Doi Number: 10.1016/j.ibiod.2004.06.010
  • Journal Name: INTERNATIONAL BIODETERIORATION & BIODEGRADATION
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.25-28
  • Keywords: biological degradation, 2, 4-dichlorophenol, Pseudomonas putida CP1
  • Dokuz Eylül University Affiliated: Yes

Abstract

Biological degradation of 2,4-dichlorophenol (DCP) by Pseudonionas putida CP1 was investigated in batch shake flask-cultures. Experiments were carried out at initial DCP concentrations between 50 and 750mg l(-1) and the rate and extent of DCP degradation were quantified. With increasing initial DCP concentrations in shake-flask cultures, percentage removal of DCP decreased and residual DCP concentrations increased proportionally. The rate of DCP degradation increased with increasing initial DCP concentration up to 577 mg DCPl(-l). Further increases in DCP concentration caused decreases in the rate of degradation because of substrate inhibition. A kinetic model based on substrate inhibition was proposed. Rate and inhibition constants were determined using the experimental data. (C) 2004 Elsevier Ltd. All rights reserved.