Macrophage Polarization Profiling in Dynamic Culture System

Yılmaz A., Özbilgiç R., Polatlı E., Erbay İ. H., SAĞ D., GÜVEN S.

Cellular and Molecular Bioengineering, cilt.18, sa.3-4, ss.311-322, 2025 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 18 Sayı: 3-4
  • Basım Tarihi: 2025
  • Doi Numarası: 10.1007/s12195-025-00863-0
  • Dergi Adı: Cellular and Molecular Bioengineering
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Applied Science & Technology Source, BIOSIS, Communication Abstracts, EMBASE, Metadex, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.311-322
  • Anahtar Kelimeler: Human macrophages, Macrophage polarization, Dynamic culture, Shear stress
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

Purpose: In this study, we aimed to develop a dynamic on-chip platform to study macrophage polarization in a more physiologically relevant way by incorporating mechanical forces which have been recently shown to play important roles in macrophage biology. Methods: We developed polymethyl methacrylate (PMMA) based platform. We examined the effects of the dynamic microenvironment on polarization states of human monocyte derived macrophages (HMDMs) towards the M1 and M2a phenotypes using lipopolysaccharide (LPS)/interferon-γ (IFN-γ) and interleukin-4 (IL-4) respectively for both static and dynamic conditions. M1 and M2 polarization levels were assessed by qPCR and flow cytometry analyses. Results: M1 and M2 polarization was achieved successfully under dynamic and static conditions. Our platform establishes that the mechanotransductive stimulation through shear stress during polarization has direct synergistic effects with stimulants on TNF-α secretion within HMDMs. Exposure to media flow rates of 0.5, 2.5, and 5 µl/min without stimulants is insufficient to induce macrophage polarization. Conclusion: The dynamic environment present inside our dynamic on-chip culture platform influences the human monocyte-derived macrophages (HMDMs) to become polarized into M1 phenotype at a greater level.