Düşük maliyetli bir fruktozamin testinin otomasyonu laboratuvar performansı ve klinikyararlılığının değerlendirilmesi

Tez Türü: Tıpta Uzmanlık

Tezin Yürütüldüğü Kurum: Dokuz Eylül Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Türkiye

Tez Danışmanı: Banu Önvural

Tezin Onay Tarihi: 2003

Tezin Dili: Türkçe

Özet:

Diabetes Mellitus (DM) is a metabolic disorder which causes destruction and failure of organs and systems in long term. Early diagnosis and strict hyperglicemia therapy of DM decreases mortality and morbidity of diabetic patients. Clinical chemistry tests are important for diagnosing and monitoring therapy in DM. Fructosamine which reflects nonenzymatic glycation of serum proteins is a usefull test to evaluate short-term glisemic control.

The aim of this study was to optimise and automate a low cost fructosamine assay and then to investigate the relationship between long-term complications. Serum fructosamine was measured by nitrobluetetrazolium method in non-diabetic controls (n=146) with a mean age of 60±20 and diabetic subjects (n=353) with a mean age of 61±11.The within-day imprecision and between day imprecision of the automated method were %0,7 (n=10) and %2,5 (n=10), respectively.

Mean fructosamine concentration were significantly increased in DM (342± 74 μmol/L) compared with control group (270±47 μmol/L) (p<0,05). This cross-sectional study suggested that fructosamine concentration was not related to long-term complications of DM (p>0,05). However, since it is highly correlated with HbA1c (r=0,817, p<0,05), fructosamine test may be a useful laboratory parameter for monitoring glycemia also being an inexpensive, appropriate for automation and relativly simple test.