The Effects of Two Different Doses of Dexmedetomidine in a Testicular Ischemia-Reperfusion Model in Rats


Bektas H., Ozzeybek D., Gunenc F., Akkus M., BAĞRIYANIK H. A., KÜME T., ...More

TURKIYE KLINIKLERI TIP BILIMLERI DERGISI, vol.32, no.3, pp.766-774, 2012 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 32 Issue: 3
  • Publication Date: 2012
  • Doi Number: 10.5336/medsci.2011-26915
  • Journal Name: TURKIYE KLINIKLERI TIP BILIMLERI DERGISI
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.766-774
  • Keywords: Dexmedetomidine, reperfusion injury, spermatic cord torsion, ENDOTOXIN-INDUCED SHOCK, INFLAMMATORY RESPONSES, TORSION/DETORSION DAMAGE, TORSION, INJURY, DURATIONS, ENDOCRINE, APOPTOSIS, MORTALITY, TESTIS
  • Dokuz Eylül University Affiliated: Yes

Abstract

Objective: Dexmedetomidine is known to have anti-inflammatory activity in both ischemic and toxic inflammatory response models. In this study, the effects of two different doses of dexmedetomidine were investigated in a rat model of testicular ischemia-reperfusion. Material and Methods: Twenty eight rats weighing 300-350 g were divided into four groups. The left testis and spermatical cord were liberated by left inguinal incision in all groups. Sham group (Group 1) (n=7) did not receive any other procedure. One hour of torsion and four hours of detorsion were applied to the other groups. The rats in ischemia/reperfusion group (Group 2) (I/R, n=7), did not receive any drug. Dexmedetomidine 50 mu g/kg for Group 3 (I/R+50D, n=7) and 100 mu g/kg for Group 4 (I/R+100D, n=7) was injected intraperitoneally after 30 mins of testicular torsion. To grade the testicular reperfusion injury tissue malondialdehyde (MDA) and nitric oxide (NO) levels were measured and the germ cell damage was evaluated histopathologically using Cosentino Scale in the excised testis. Results: MDA levels in group I/R were significantly higher than in sham, I/R+50D and I/R+100D groups (p<0.001). MDA levels in I/R+50D and I/R+100D were also significantly different (p=0.01). NO levels were significantly higher in group I/R when compared to Sham, I/R+50D and I/R+100D groups (p=0.003). Hystopathological scores were higher in I/R group than in I/R+50D and I/R+100D groups (p=0.001). Conclusion: Deksmedetomidine treatment at a dose of 50 and 100 mu g/kg, attenuated reperfusion injury in a dose-dependent manner in this experimental testicular ischemia reperfusion model.