The effect of lipoic acid on wound healing in a full thickness uterine injury model in rats


CİLAKER MIÇILI S., GÖKER A., SAYIN O., Akokay P., Ergur B. U.

JOURNAL OF MOLECULAR HISTOLOGY, vol.44, no.3, pp.339-345, 2013 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 44 Issue: 3
  • Publication Date: 2013
  • Doi Number: 10.1007/s10735-013-9485-8
  • Journal Name: JOURNAL OF MOLECULAR HISTOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.339-345
  • Keywords: Lipoic acid, Rat, Uterine scar, Full thickness uterine injury, VEGF, SMA, ENDOTHELIAL GROWTH-FACTOR, ANGIOGENESIS, REGENERATION, EXPRESSION, VEGF
  • Dokuz Eylül University Affiliated: Yes

Abstract

Aim of this study was to investigate the effects of lipoic acid on uterine wound healing by immunohistochemical and biochemical assay in a rat uterine horn model with full thickness injury. Thirty-two female Wistar albino rats were randomised into five groups: Control group, with no intervention; uterine scar group 15days (US15d), uterine scar group 15 days + alpha lipoic acid (ALA) (US15d + ALA), uterine scar group 30 days (US30d) and uterine scar group 30 days + ALA (US30 days + ALA). After uterine incision 100 mg/kg of ALA was administered by oral gavage for either 15 or 30 days. Vascular endothelial growth factor (VEGF) and alpha smooth muscle actin (alpha-SMA) distribution were evaluated by immunohistochemical methods in tissue and ELISA methods in tissue homogenate. The percentage of alpha-SMA positive area in US15d + ALA and US30d + ALA groups was significantly higher than US15 and US30d groups. The percentage of VEGF positive area in US15d + ALA group was significantly higher than US15d group and US30d + ALA group was significantly higher than US30d group. Biochemically, alpha-SMA was significantly higher in the US15d + ALA group when compared to US15d group and higher in US30d + ALA group when compared to US30d group. VEGF was significantly higher in US15d + ALA and US30d + ALA groups when compared to US15 and US30d groups. In conclusion, ALA was found to be effective in enhancing wound healing in uterine full thickness injury.