Transforming growth factor β receptor II mutations in sporadic colon cancer and bilateral breast cancer

KIZILDAĞ S., Sercan Z., ÖZER E., Sakizli M.

Journal of B.U.ON., cilt.6, sa.1, ss.75-77, 2001 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 6 Sayı: 1
  • Basım Tarihi: 2001
  • Dergi Adı: Journal of B.U.ON.
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.75-77
  • Anahtar Kelimeler: Bilateral breast cancer, Colorectal cancer, Microsatellite instability, Mutation, TGFβ-RII
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

Purpose: The aim of this study was to further clarify the role of transforming growth factor β receptor II (TGFβ-RII) alterations in randomly selected sporadic colon and bilateral breast cancer. Materials and methods: 17 sporadic colorectal cancer and 26 bilateral breast cancer archive specimens were analyzed for mutations in the adenine 10 (A10) simple repeat sequences of the TGFβ-RII gene. Following DNA isolation, the polyadenin region in the TGFβ-RII gene was amplified by polymerase chain reaction (PCR). The amplification products were run on polyacrylamide gels (with 8M urea) and visualized by silver staining. Corresponding normal tissue specimens binding patterns were used as controls. Results: A10 mutations were not detected in any of the colorectal cancer samples and only 1 (3.84%) out of the 26 bilateral breast cancer samples showed allelic shift representing genomic instability in this region. Conclusion: Mutation of the TGFβ-RII gene may contribute to cancer pathogenesis in tumors exhibiting microsatellite instability (MI), but, in general, it does not seem to play a major role in epithelial carcinogenesis other than in this specified group of tumors.