Investigation of the effect of fluconazole on nitrite production by murine macrophages


Gulay Z., Yucesoy M., Yulug N.

10th Mediterranean Congress of Chemotherapy, Antalya, Türkiye, 20 - 25 Ekim 1996, ss.149-153 identifier

  • Yayın Türü: Bildiri / Tam Metin Bildiri
  • Cilt numarası:
  • Basıldığı Şehir: Antalya
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.149-153
  • Dokuz Eylül Üniversitesi Adresli: Hayır

Özet

Azole group of antifungal agents exert their activity by binding to and inhibiting the activities of a cytochrome(cy) P 450 enzyme. Because of the functional and sequence homologies of cy P450 enzmes and nitric oxide synthases, we investigated the effect of fluconazole, a triazole, on nitric oxide (NO) production. Fluconazole was added to mouse peritoneal adherent cell cultures at varying doses 1 or 4 days prior to lipopolysaccharide (LPS) stimulation. NO response was measured by nitrite accumulation in the supernatants. Nitrile production was inhibited by 18.3% (p>0.05), 5.6% (p>0.05) and 2.8% (p>0.05) in the presence of 100 mu g/ml, 10 mu g/ml and 0.5 mu g/ml fluconazole, respectively. When exposure to the agent was extended to 4 days, this inhibitory effect increased to 25.4% (p<0.05) at 100 mu g/ml and 10.89% (p>0.05) at 10 mu g/ml. The results of the present study indicate that, short exposure to fluconazole modulates nitrite production only minimally at pharmacological doses.