The effect of Misoprostol, a prostaglandin E1 analog, on apoptosis in ischemia-reperfusion-induced intestinal injury

Topcu I., Vatansever S., Var A., Cavus Z., Cilaker S., Sakarya M.

ACTA HISTOCHEMICA, cilt.109, sa.4, ss.322-329, 2007 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 109 Sayı: 4
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1016/j.acthis.2006.10.007
  • Dergi Adı: ACTA HISTOCHEMICA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.322-329
  • Anahtar Kelimeler: ischemia-reperfusion, misoprostol, prostaglandin E1, ileum, apoptosis, rats, NITRIC-OXIDE SYNTHASE, DYSFUNCTION, SUPEROXIDE, PREVENTION, ASSAY
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

The aim of this study was to investigate whether Misoprostol, a synthetic prostaglandin (PG) E1 analog, has any effect on the prevention of apoptosis in ischemia-reperfusion (I/R)-induced intestinal injury. Thirty adult mate Wistar albino rats were divided into three groups: group I = sham operated+satine; group II = I/R+saline; and group III = I/R+Misoprostol. Misoprostol. (50 mu g/kg/d) was administered as an intragastric meat for 3 days. The terminal ileum was collected for histological and biochemical. investigations. Apoptotic cells were detected by terminal deoxynucteotidyl transferase-mediated dUTP nick end-labetted (TUNEL) reaction. Immunohistochemical. analysis was performed to determine the distribution of inducible nitric oxide synthase (iNOS) and endothelial NOS (eNOS). Samples were also analyzed for matondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px). The number of TUNEL-positive cells was higher in group II when compared to the other two groups (p < 0.05). In group III this value was higher when compared to group I, but lower than group II (p<0.05). iNOS immunoreactivity was not detected in ileum sections of group I animals, but moderate immunoreactivity was seen in group II and mild immunoreactivity in group III. The immunoreactivity of eNOS was moderate in ileum sections of all three groups. In ileum tissue, MDA was found to be higher in group II compared to group I (p<0.05), but there was no difference in group III. SOD was not different between groups I and III, but was significantly higher in group II (p<0.05). In our experimental model of I/R-induced intestinal injury, apoptosis is induced in enterocytes, whereas Misoprostol decreases enterocyte apoptosis in this experimental model. Our results indicate that Misoprostol may play a key rote in the pathophysiologic events leading to failure of the intrinsic gut barrier defense mechanisms of intestinal epithelium. (c) 2007 Elsevier GmbH. All rights reserved.