Grape seed extract has superior beneficial effects than vitamin E on oxidative stress and apoptosis in the hippocampus of streptozotocin induced diabetic rats

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Yonguc G. N., Dodurga Y., Adıgüzel E., Gundogdu G., Küçükatay V., Özbal S., ...Daha Fazla

GENE, cilt.555, sa.2, ss.119-126, 2015 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 555 Sayı: 2
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1016/j.gene.2014.10.052
  • Dergi Adı: GENE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.119-126
  • Anahtar Kelimeler: Diabetes, Grape seed extract, Vitamin E, Hippocampus, Oxidative stress, Apoptosis, NF-KAPPA-B, GLUCOSE-INDUCED APOPTOSIS, RETINAL GANGLION-CELLS, PROANTHOCYANIDIN EXTRACT, COGNITIVE DYSFUNCTION, PROCYANIDIN EXTRACT, CEREBRAL-ISCHEMIA, SCIATIC-NERVE, DNA-DAMAGE, PC12 CELLS
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

We aimed to investigate the effects of grape seed extract (GSE) and vitamin E (Vit E) on oxidative stress and apoptosis in the hippocampus of streptozotocin-induced diabetic rats. In Control, Diabetic, and Diabetic treated with GSE (Diabetic + GSE) and vitamin E (Diabetic + Vit E) groups, oxidative stress index (OSI), TUNEL staining and Bcl-2, Bcl-XL, Bax, caspase-3, -9, and -8, Cyt-c, TNF-alpha, and NF-kappa B gene expressions were evaluated. OSI was significantly increased in the plasma and hippocampus of the Diabetic compared to Control group and decreased in Diabetic + GSE and Diabetic + Vit E groups compared to Diabetic. TUNEL positive neurons significantly increased in the hippocampus of the Diabetic group compared to Control and decreased in Diabetic + GSE (more prominently) and Diabetic + Vit E groups compared to Diabetic. In the hippocampus of the Diabetic group, Bcl-2 and Bcl-XL gene expressions were significantly decreased; Bax, caspase-3, -9, and -8, Cyt-c, TNF-alpha, and NF-kappa B gene expressions were significantly increased compared to Control. In Diabetic + GSE and Diabetic + Vit E groups, Bcl-2 gene expressions were significantly increased; Bcl-XL gene expressions did not differ compared to the Diabetic group. The expression of Bax, caspase-3, -9, and -8, Cyt-c, TNF-alpha, and NF-kappa B genes in the Diabetic + GSE group and the expression of caspase-3 and -9, TNF-alpha, and NF-kappa B genes in the Diabetic + Vit E group were significantly decreased compared to Diabetic. In conclusion, GSE (more prominently) and vitamin E decreased oxidative stress and neuronal apoptosis occurring in the hippocampus of diabetic rats. (C) 2014 Elsevier B.V. All rights reserved.