Batch production of Pyranose 2-oxidase from Trametes versicolor (ATCC 11235) in medium with a lignocellulosic substrate and enzymatic bleaching of cotton fabrics

Pazarlioglu N. K., ERDEN KOPAR E., Ucar M. C., AKKAYA A., SARIIŞIK A. M.

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, cilt.28, sa.4, ss.1523-1531, 2012 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 28 Sayı: 4
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1007/s11274-011-0955-4
  • Dergi Adı: WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1523-1531
  • Anahtar Kelimeler: Pyranose 2-oxidases, Trametes versicolor (ATCC 11235), Agricultural wastes, Lignin degradation, Submerged fermentation, Cotton fabric, PHANEROCHAETE-CHRYSOSPORIUM, MANGANESE PEROXIDASE, OXIDASE, IMPROVE, ENZYMES, LACCASE, PURIFICATION, DEGRADATION, WASTES
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

The aim of this work was to determine new, different and low-cost substrates that can be used for enzyme production from the white rot fungus Trametes versicolor (ATCC 11235) by taking advantage of the broad substrate specificity of pyranose 2-oxidase. In this report, we investigated the production of pyranose 2-oxidase from T. versicolor (ATCC 11235) using ten different agricultural residues such as clover straw, almond shells, hazelnut cobs, grass and others. Pyranose 2-oxidase activity was determined as 2.332 U/g at the 9th day in a submerged culture containing clover straw and tap water shaken at 150 rpm and 26A degrees C, and the optimum clover straw concentration was determined to be 12 g/l. The effects of different glucose, nitrogen and phosphate sources on the production of pyranose 2-oxidase were studied in the clover straw medium. Analyses of biomass, protein, reduced sugar and nitrogen concentrations were also monitored in a clover straw medium that did not contain carbon or nitrogen and phosphate sources under the parameters determined. The produced pyranose 2-oxidase was used for improving the properties of cotton fabrics.