Complete genome sequence of a novel mitovirus from the ectomycorrhizal fungus Geopora sumneriana


Sahin E., AKATA I.

ARCHIVES OF VIROLOGY, vol.164, no.11, pp.2853-2857, 2019 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 164 Issue: 11
  • Publication Date: 2019
  • Doi Number: 10.1007/s00705-019-04367-x
  • Journal Name: ARCHIVES OF VIROLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.2853-2857
  • Dokuz Eylül University Affiliated: No

Abstract

A double-stranded RNA (dsRNA) segment was extracted from the ectomycorrhizal fungus Geopora sumneriana (Cooke) M. Torre, and its full-length cDNA sequence, comprising 3146 nucleotides, was determined. Sequence analysis revealed the presence of a large open reading frame (ORF) on the positive strand of this dsRNA segment when the mold mitochondrial genetic code was applied. The ORF encodes a putative RNA-dependent RNA polymerase (RdRp), which shares the highest degree of similarity with Tuber excavatum mitovirus, with 37.52% identity. This dsRNA segment represents the genome replication intermediate of a novel mitovirus that was tentatively designated as "Geopora sumneriana mitovirus 1" (GsMV1). Phylogenetic analysis further suggested that GsMV1 is a member of the family Narnaviridae. This is the first study reporting on a mitovirus genome sequence in the ectomycorrhizal fungus G. sumneriana.