Effects of nebulized sildenafil on lung histology, oxygen saturation and blood gas parameters in asthma attack of BALB/c mice Asti{dotless}m ataǧi{dotless}ndaki BALB/c farelerde nebulize sildenafilin akciǧer histolojisi, oksijen saturasyonu ve kan gazi{dotless} parametreleri üzerine etkileri


Erge D., Karaman Ö., Babayiǧit Hocaoǧlu A., Erbil G., Yilmaz O., BAĞRIYANIK H. A., ...More

Turkiye Klinikleri Journal of Medical Sciences, vol.33, no.4, pp.1133-1142, 2013 (Scopus) identifier

  • Publication Type: Article / Article
  • Volume: 33 Issue: 4
  • Publication Date: 2013
  • Doi Number: 10.5336/medsci.2012-33154
  • Journal Name: Turkiye Klinikleri Journal of Medical Sciences
  • Journal Indexes: Scopus
  • Page Numbers: pp.1133-1142
  • Keywords: Asthma, Mice inbred BALB C, Microscopy electron, Phosphodiesterase 5 inhibitors
  • Dokuz Eylül University Affiliated: Yes

Abstract

Objective: Sildenafil is a phosphodiesterase 5 inhibitor which is used for the treatment of erectile dysfunction. Inhibition of phosphodiesterase 5 promotes alveolar growth and angiogenesis, and attenuates inflammation and airway reactivity in animal models. We aimed to investigate the effects of nebulized sildenafil in asthma attack. Material and Methods: In all mice, except Group 1 (control), chronic asthma was established with repeated intraperitonal and aerosolized ovalbumin administrations. In three groups of chronic asthmatics (Group 3, 4 and 5), asthma attack was developed with increasing doses of methacoline inhalation. Afterwards,s nebulized sildenafil (0.07 mg/ml), nebulized budesonide (0.25mg/ml) and nebulized saline were given to Group 3, 4 and 5, respectively. Group 1 and Group 2 were not treated with either methacholine or nebulized therapies. Heart rates and oxygen saturations were measured. Blood gas analyses were performed and lung histologies were examined by light and electron microscopies. Results: Thicknesses of basement membrane, subepithelial smooth muscle layer and epithelium and numbers of mast and goblet cells were significantly lower in control group (Group 1) compared to the chronic asthmatics (Group 2) (p<0.001, p=0.002, p=0.001, p<0.001, p<0.001, respectively). These results suggested that the model of chronic asthma was successfully established. Numbers of mast cells were significantly lower in sildenafil group compared to the saline group (p=0.020). Oxygen saturation, heart rate, and blood gas parameters did not show differences between the treatment groups. Conclusion: Nebulized sildenafil significantly decreased the numbers of mast cells compared to placebo. Nevertheless, nebulized sildenafil was nor effective on oxygen saturation, heart rate, and blood gas parameters. © 2013 by Türkiye Klinikleri.