Radiolabeling, In Vitro Cell Uptake, and In Vivo Photodynamic Therapy Potential of Targeted Mesoporous Silica Nanoparticles Containing Zinc Phthalocyanine

Er O., Tuncel A., Ocakoğlu K., Ince M., Kolatan H. E., Yılmaz O., ...Daha Fazla

MOLECULAR PHARMACEUTICS, cilt.17, sa.7, ss.2648-2659, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 17 Sayı: 7
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1021/acs.molpharmaceut.0c00331
  • Dergi Adı: MOLECULAR PHARMACEUTICS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Biotechnology Research Abstracts, Chemical Abstracts Core, EMBASE, MEDLINE
  • Sayfa Sayıları: ss.2648-2659
  • Anahtar Kelimeler: Zn(II) phthalocyanine, mesoporous silica nanoparticles, cetuximab, singlet oxygen, photodynamic therapy, PANCREATIC-CANCER, ANTIBODY, GEMCITABINE, CETUXIMAB, BIODISTRIBUTION, EXCRETION, DELIVERY, SIZE, BIOCOMPATIBILITY, PHOTOSENSITIZERS
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

Photodynamic therapy (PDT) is a noninvasive therapy based on the photodynamic effect. In this study, we sought to determine intracellular uptake and in vivo photodynamic therapy potential of Zn phthalocyanine-loaded mesoporous silica nanoparticles (MSNPS) against pancreatic cancer cells. MSNPS were labeled with I-131; the radiolabeling efficiency was found to 95.5 +/- 1.2% in pH 9 and 60 min reaction time. Besides, the highest intracellular uptake yields of I-131-MSNPS nanoparticles in MIA PaCa-2, AsPC-1, and PANC-1 cells were determined as 43.9 +/- 3.8%, 41.8 +/- 0.2%, and 37.9 +/- 1.3%, respectively, at 24 h incubation time. In vivo PDT studies were performed with subcutaneous xenograft cancer model nude mice with AsPC-1 pancreatic cancer cells. For photodynamic therapy, 685 nm red laser light 100 J/cm(2) light dose using and 5-20 mu M ZnPc containing MSNPS concentrations were applied. Histopathological studies revealed that the ratio of necrosis in tumor tissue was higher in the treatment group than the control groups.