A novel biorecognition receptor Citropin-A modified impedimetric biosensor for detection of LNCaP prostate cancer cells

Fedaci C., ERTUĞRUL UYGUN H. D., UYGUN Z. O., AKÇAY Y.

ANALYTICAL BIOCHEMISTRY, cilt.652, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 652
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1016/j.ab.2022.114772
  • Dergi Adı: ANALYTICAL BIOCHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, Compendex, EMBASE, MEDLINE, Veterinary Science Database
  • Anahtar Kelimeler: Impedance, Chronoimpedance, Biosensor, Antimicrobial peptide, Prostate cancer, LABEL-FREE DETECTION
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

In this study, Citropin-A (Cit-A) as a biorecognition receptor was used for the first time to develop electro-chemical impedance spectroscopy (EIS) based biosensor for the detection of Lymph Node Carcinoma of the Prostate (LNCaP) cancer cells. The biosensor was engineered by modification of a gold electrode (AuE) with cysteamine (Cys), Poliamidoamin (PAMAM (G4)) dendrimers, avidin, and biotinylated Cit-A, respectively. The detection time of the LNCaP cells was determined as 300 s by chronoimpedance (CI). Chronoimpedance also provided an exact detection time to avoid non-specific adsorptions. The biosensor showed good linearity between 1500 cells/L and 12000 cells/L, limit of detection (LOD), and limit of quantification (LOQ) values were 518 cells/ L and 1570 cells/L, respectively.