Three-dimensional silk impregnated HAp/PHBV nanofibrous scaffolds for bone regeneration

ÇOLPANKAN GÜNEŞ O., Unalan I., ÇEÇEN B., ZİYLAN A., HAVITÇIOĞLU H., Ustun O., ...Daha Fazla

INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS, cilt.68, sa.5, ss.217-228, 2019 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 68 Sayı: 5
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1080/00914037.2018.1443928
  • Dergi Adı: INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.217-228
  • Anahtar Kelimeler: Bone tissue engineering, hydroxyapatite, PHBV, silk fibroin, wet-electrospinning, IN-VIVO, TISSUE, FIBROIN, FABRICATION, NANOCOMPOSITE, BIOMATERIALS, FIBERS, ACID)
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

Three-dimensional silk fibroin impregnated poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanofibrous scaffolds with or without hydroxyapatite (HAp) were prepared by wet-electrospinning method followed by freeze-drying. Scaffolds with cotton wool-like structure have the average fiber diameter of 450-850 nm with 80-85% porosity. In-vitro cell culture tests using MG-63 osteosarcoma human cells revealed improved cell viability, alkaline phosphatase (ALP) activity and total cellular protein amount on the silk impregnated scaffolds compared to PHBV and HAp/PHBV scaffolds after 10 days of cell culture. Immunohistochemical analyses on the silk impregnated scaffolds showed that HAp triggered cell penetration and type I collagen production. Besides, HAp mineralization tendency increased with a decrease in percent crystallinity of the scaffolds comprising HAp and silk after 4 weeks of incubation in simulated body fluid. Consequently, cotton wool-like HAp/PHBV-SF scaffold would be a promising candidate as a bone-filling material for tissue regeneration.