Akademik Veri Yönetim Sistemi
REVISTA CIENTÍFICA. FACULTAD DE CIENCIAS VETERINARIAS. LUZ, cilt.32, ss.1-7, 2022 (SCI-Expanded)
The study aims to identify phylogenetic groups and antibiotic
susceptibility of poultry Escherichia coli (APEC) isolates. E. coli was
phenotypically and biochemically characterized. Isolates from 8/30
(26.66%) liver, 7/30 (23.33%) heart, and 4/30 (13.33%) spleen of 37-42
days old vaccinated broiler chickens were assessed. Then the E. coli
isolates (19/90; 21.11%) were phylogrouped by quadruplex genotyping
based on the presence or absence of arpA, chuA, yjaA genes, and
TspE4.C2 DNA fragment.The majority of APEC strains belonged to
phylogenetic group C, followed by groups A, E, and F. Phylogroup C
was observed in the liver, phylogroup A in both liver and heart samples,
phylogroup E in the heart and spleen, and phylogroup F in the liver. The
highest antibiotic resistance was observed in Amoxicillin-Clavulanic
acid and Ampicillin (100%) predominantly in groups A and E according
to antibacterial susceptibility tests. Multiple antibiotic resistance
(MDR) for APEC strains was also found at 68.42% (13/19). Of the 19
isolates tested, only 13 (68%) were susceptible to high levels of
gentamicin. APEC strains belonging to phylogroups C, A, and E are
of epidemiological importance for broilers. It would be beneficial to
investigate new phylogroups by performing more detailed genotypic
analyzes in APEC strains.