Comparative Analysis of SSR Marker Diversity with Maize Populations and Their Applications to Genetic Studies


Alaca Yıldırım E., Matur F., Songur U., Kahrıman F.

Ecology and Evolutionary Biology Symposium 2024, Çanakkale, Türkiye, 23 - 25 Temmuz 2024, ss.1-2

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: Çanakkale
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.1-2
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

Maize, a critical field crop, garners significant attention in scientific circles owing to its diverse global applications and pivotal role in human and animal nutrition. This study aims to delve into specific indices of studied SSR markers and their implications for genetic studies among maize populations. Twenty SSR primers and six local populations and two commercial hybrid lines of maize were employed to assess key traits such as heterozygosity (h), polymorphic information content (PIC), effective multiplex ratio (EMR), marker index (MI), and resolution power (PR) across various populations. h reflects marker diversity, ranging from 0.32 to 0.86 indicating varying diversity. PIC indicates marker informativeness, ranging values from 0.268 to 0.844; notably, eleven markers indicate high informativeness (PIC>0.5). EMR demonstrates the markers' ability to detect differences, ranging from 0.5 to 3.125. MI offers an overall evaluation of marker system utility, varying from 0.134 to 3.379, while PR measures the markers' capacity to distinguish between different genotypes, with values ranging from 0.667 to 6.25. Moreover, the Fst value (Fst: 0.462) suggests significant population differentiation, supported by Jaccard similarity indices which gives similarity result between two populations based on presence of alleles. Jaccard results, ranges from 0.1712 to 0.274, emphasizing the distinct genetic compositions of each group. As we expected, the comparison of two hybrid lines and local populations showed less similarity compared to local population comparisons. These insights offer valuable guidance for future genetic studies in maize populations. This study is derived from Ezgi Alaca Yıldırım's thesis study.