Wet-electrospun PHBV nanofiber reinforced carboxymethyl chitosan-silk hydrogel composite scaffolds for articular cartilage repair

ÇOLPANKAN GÜNEŞ O., ZİYLAN A., Tasdemir S., ŞENDEMİR A.

JOURNAL OF BIOMATERIALS APPLICATIONS, cilt.35, ss.515-531, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 35
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1177/0885328220930714
  • Dergi Adı: JOURNAL OF BIOMATERIALS APPLICATIONS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Aquatic Science & Fisheries Abstracts (ASFA), Biotechnology Research Abstracts, Communication Abstracts, Compendex, EMBASE, INSPEC, MEDLINE, Metadex, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.515-531
  • Anahtar Kelimeler: Cartilage regeneration, fiber reinforced composite scaffold, wet-electrospinning, PHBV, silk fibroin, carboxymethyl chitosan, TEXTURE PROFILE ANALYSIS, TISSUE, BONE, FIBROIN, VISCOELASTICITY, BIOMATERIALS, DESIGN, FIBERS
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

The objective of the study was to produce three-dimensional and porous nanofiber reinforced hydrogel scaffolds that can mimic the hydrated composite structure of the cartilage extracellular matrix. In this regard, wet-electrospun poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanofiber reinforced carboxymethyl chitosan-silk fibroin (PNFs/CMCht-SF) hydrogel composite scaffolds that were chemically cross-linked by poly(ethylene glycol) diglycidyl ether (PEGDE) were produced. To the best of our knowledge, this is the first study in cartilage regeneration where a three dimensional porous spongy composite scaffold was obtained by the dispersion of wet-electrospun nanofibers within a polymer matrix. All of the produced hydrogel composite scaffolds had an interconnected microporous structure with well-integrated PHBV nanofibers on the pore walls. The scaffold comprising an equal amount of PEGDE and polymer (PNFs/CMCht-SF1:PEGDE1) demonstrated comparable water content (91.4 +/- 0.7%), tan delta (0.183 at 1 Hz) and compressive strength (457 +/- 85 kPa) values to that of articular cartilage. Besides, based on the histological analysis, this hydrogel composite scaffold supported the chondrogenic differentiation of bone marrow mesenchymal stem cells. Consequently, this hydrogel composite scaffold presented a great promise for cartilage tissue regeneration.