TERMIS EU 2019, Rodos, Yunanistan, 27 - 31 Mayıs 2019, ss.1188
Novel Helix
Aspersa extract loaded Chitosan Scaffolds for Hard Tissue Regeneration
M. Perpelek1,
S. Tamburaci2, F. Tihminlioglu2, A. Karakasli1,
H. Havitcioglu1
Presenting Author: Merve
Perpelek, merveperpelek@gmail.com
1Instıtute of Healt
Science, Department of Biomechanics, Dokuz Eylul University,
Narlidere,
İzmir, Turkey,
2İzmir Institute of
Technology, Department of Chemical Engineering, Gulbahce Campus, Urla, İzmir,
Turkey
INTRODUCTION: Cartilage
damage is a highly observed clinical situation in orthopaedic treatments.
Although there are traditional treatments known as autografting and allografting
techniques, they have risk factors such as donor site morbidity, infections and
immunogenic reactions[1,2]. Recently, studies have focused on tissue
engineering applications as alternative methods to replace surgical procedures.
In this study, it was aimed to fabricate bioactive Helix aspersa extract loaded chitosan scaffolds
with slime and mucus incorporation and to investigate the composite structures in
terms of physical, morphological and biological compatibility for
bone-cartilage regeneration.
METHODS: Chitosan-slime and chitosan-mucus scaffolds were prepared
with snail (Helix aspersa) secretions at different amounts (0.5%, 1% and 3%).
Scaffolds were fabricated via lyophilization method. Morphology of scaffolds
were determined by Scanning Electron Microscopy (SEM) and chemical composition
by Fourier Transform Infrared Spectroscopy (FT-IR). In addition, swelling ratio
test, open porosity determination, biodegradation test, biomechanical analysis
(compression tests) and antimicrobial tests for five different strains were
performed. In vitro bioactivity of chitosan-slime and chitosan-mucus scaffolds
has been investigated with Saos-2 and SW 1353 proliferation. Osteogenic effect
of extracts is investigated with ALP activity, osteocalcin secretion and biomineralization,
whereas, chondrogenic potential of scaffolds is evaluated with GAG assay,
hydroxyproline content and COMP assay.
DISCUSSION & CONCLUSIONS: The scaffolds which have produced for bone
and cartilage provided antimicrobial characteristics as well as suitable
morphological and mechanical properties.
ACKNOWLEDGEMENTS: The authors are greatful to Izmir Institute of
Technology (Iztech) Biotechnology and Bioengineering Research and Application
Center (IZTECH BIOMER) for antimicrobial tests and FT-IR analysis, Center for
Material Research (IZTECH CMR) for SEM imaging.
REFERENCES
[1] Temenoff JS et al. Biomaterials 2000;21:431-34
[2] O’Driscoll SW J
Bone Joint Surg Am. 1998;80:1795-812.