Akademik Veri Yönetim Sistemi
PLASMONICS, cilt.19, sa.2, ss.753-764, 2024 (SCI-Expanded, Scopus)
Sexually transmitted infections (STIs) due to Neisseria gonorrhoeae (NG) and Chlamydia trachomatis (CT) pose global health challenges due to their widespread prevalence through sexual contact. Rapid and user-friendly diagnostic solutions are crucial to curb their spread and mitigate potential complications. In this article, we introduced a portable plasmonic biosensor kit for the detection of NG and CT bacteria in urine samples. Our platform merges label-free plasmonic nanohole sensing with lens-free computational imaging, revolutionizing point-of-care pathogen diagnosis. The system captures and analyzes diffraction field images, uncovering subtle bacterial presence cues. With a low-cost pump compartment and flow cell, we ensured precise sample delivery to the plasmonic chip. The system software controls hardware setup, test execution, and image analysis for bacterial detection. Leveraging control and sensor antibodies, our technology excels in sensitivity and selectivity. Microfluidic channels with multiple sensor locations enable separate NG and CT testing. Our imaging-based approach allows simultaneous detection, obviating the need for separate assays. Integrating multiple sensor sites ensures robust high-throughput testing. Employing plasmonic pixels for control tests effectively mitigates the background noise. Simple optics, affordable components, and controlled environment underscore the practicality of our handheld biosensor. Strong detection limits, e.g., 452 CFU/mL for NG and 121 CFU/mL for CT bacteria (CFU: colony-forming unit), position our technology as a transformative point-of-care diagnostic tool for pathogenic diseases, bridging accessibility and affordability gaps.