The effect of erythropoietin on S100 protein expression in cochlea after acoustic overstimulation: An experimental study Eritropoetinin gürültü uygulaması sonrası kokleada s100 proteini ekspresyonuna etkisi: Deneysel Çalışma


Gürgen S. G., Gürgen O., KIRKIM G., KOLATAN H. E., GÜRKAN S., Eskiizmir G.

Journal of Clinical and Analytical Medicine, vol.6, no.3, pp.304-308, 2015 (ESCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 6 Issue: 3
  • Publication Date: 2015
  • Doi Number: 10.4328/jcam.2009
  • Journal Name: Journal of Clinical and Analytical Medicine
  • Journal Indexes: Emerging Sources Citation Index (ESCI), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.304-308
  • Keywords: Cochlea, Acoustic Trauma, Erythropoietin, S100, Immunohistochemistry, SENSORINEURAL HEARING-LOSS, GUINEA-PIG, AUDITORY-NERVE, NOISE, NEURONS, APOPTOSIS, EXPOSURE, DEAFNESS, RECEPTOR, INJURY
  • Dokuz Eylül University Affiliated: Yes

Abstract

© 2015, Journal of Clinical and Analytical Medicine. All rights reserved.Aim: To investigate the effect of Erythropoietin on acoustically overstimu-lated rat spiral ganglion neurons (SGNs) using S100 protein immunostaining. Material and Method: Twenty-two Wistar albino rats were divided into three groups: healthy control group (n=7), Saline solution (n=7) and Erythropoietin injection groups (n=8). Saline solution and Erythropoietin injection groups re-ceived white noise (100 dB SPL) for 3 hours. Cochlear sections were stained by silver staining technique and immunostained by S100 antibody. Results: Histochemical analysis of silver staining sections revealed normal structure and a weak staining in SGNs of healthy control group. However, dark-black cytoplasmic staining, cellular shrinkage and degeneration were detected in saline injection group. On the other hand, a few weakly stained neurons were observed in erythropoietin injection group. S100 staining demonstrated strong reaction in Schwann cells and myelin sheaths of SGNs in healthy con-trol group (p<0.05). In saline solution injection group, Schwann cells showed moderate S100 reaction and other regions of SGNs showed weak reaction (p<0.05). In erythropoietin injection group, strong S100 expression almost similar to the healthy control group was determined, although there was an occasional decrease. Discussion: Erythropoetin may prevent noise induced SGN degeneration via protecting the Schwann cells in rat cochlea.