H2A.Z is dispensable for both basal and activated transcription in post-mitotic mouse muscles

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Belotti E., Lacoste N., Simonet T., Papin C., Padmanabhan K., Scionti I., ...Daha Fazla

NUCLEIC ACIDS RESEARCH, cilt.48, sa.9, ss.4601-4613, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 48 Sayı: 9
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1093/nar/gkaa157
  • Dergi Adı: NUCLEIC ACIDS RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, Applied Science & Technology Source, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, EMBASE, MEDLINE, Veterinary Science Database, Directory of Open Access Journals, DIALNET
  • Sayfa Sayıları: ss.4601-4613
  • Dokuz Eylül Üniversitesi Adresli: Hayır

Özet

While the histone variant H2A.Z is known to be required for mitosis, it is also enriched in nucleosomes surrounding the transcription start site of active promoters, implicating H2A.Z in transcription. However, evidence obtained so far mainly rely on correlational data generated in actively dividing cells. We have exploited a paradigm in which transcription is uncoupled from the cell cycle by developing an in vivo system to inactivate H2A.Z in terminally differentiated post-mitotic muscle cells. ChIP-seq, RNA-seq and ATAC-seq experiments performed on H2A.Z KO post-mitotic muscle cells show that this histone variant is neither required to maintain nor to activate transcription. Altogether, this study provides in vivo evidence that in the absence of mitosis H2A.Z is dispensable for transcription and that the enrichment of H2A.Z on active promoters is a marker but not an active driver of transcription.