The effect of a single dose of Mk-801 use on adult brain tissue after an experimental head trauma model applied in immature rats


Çiğel A., SAYIN O., Gürgen S. G., SÖNMEZ A.

Neurological Research, vol.47, no.2, pp.105-114, 2025 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 47 Issue: 2
  • Publication Date: 2025
  • Doi Number: 10.1080/01616412.2024.2448633
  • Journal Name: Neurological Research
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS
  • Page Numbers: pp.105-114
  • Keywords: blunt head trauma, hippocampus, MK-801, Nestin, CD133, neuronal development
  • Dokuz Eylül University Affiliated: Yes

Abstract

Objective: Within the scope of this research, the long-term effects of experimental blunt head trauma on immature rats and MK-801 administered acutely after trauma on the brain tissue will be examined. In addition, the impact of trauma and MK-801 on Nestin and CD133, which are essential stem cells, will be evaluated by immunohistochemical and ELISA methods. Methods: In this study, the contusion trauma model was used. Sprague Dawley rats 30 7-day-old were divided into three groups: Group 1 (n = 10) control group, Group 2 (n = 10) trauma Group (head trauma applied), and Group 3 (n = 10) MK-801 + trauma Group. In the third group, immediately after head trauma, MK-801 (Sigma M107) dissolved in physiological saline was administered as a single dose of 1 mg/kg ip. Results: The concentration of nestin was significantly higher in the control group compared to both the trauma and trauma+drug groups (p < 0.001). CD133 was statistically significantly higher in the control group compared to the other two groups (p = 0.002). It was determined that the differences in Nestin CA1 and DG measurements resulted from the trauma and control and trauma and trauma+drug groups, and the differences in CD133 CA1 and DG measurements resulted from the trauma and control group. Conclusion: The positive effect of MK-801 on neuroprotective and neuronal proliferation was elaborated. Administration of MK-801 significantly induced nestin and CD133 concentrations in the injured tissue.