İzmir-Kiraz İlçesinde Üretilen Silaj ve Sütlerde Mikotoksin Varlığının Saptanması ve Bazı Kan Parametrelerine Etkileri

Çabuk M. (Executive), Polat Dinçer P. F., Semen Z., Nayir M. B., Nizam M. Y., Alçiçek A., et al.

Project Supported by Higher Education Institutions, 2022 - 2024

  • Project Type: Project Supported by Higher Education Institutions
  • Begin Date: August 2022
  • End Date: April 2024

Project Abstract

Silage is a roughage obtained as a result of fermentation of green forage with high water content by lactic acid bacteria under anaerobic conditions. Corn silage is one of the most important animal feeds in many countries around the world as well as in our country. Silages that are not made by the proper and hygienic rules may cause mycotoxin-producing fungi (fungi) to contaminate the silage create a breeding environment and cause mycotoxin accumulation. The microbiological feature of feed raw materials can cause economic losses as well as affect human health through animal health and animal products obtained from these animals. Therefore, the determination of these mycotoxins in feeds and periodic controls is gaining importance day by day.

With this research project, corn silage samples were collected from 45 dairy farms operating in the Kiraz district, where Dokuz Eylül University Faculty of Veterinary Medicine is located, and in these samples, Aflatoxin B1 (AFB1), Deoxynivalenol (DON), Zearalenone (ZEA), Fumonisin (FUMO), which cause mycotoxicosis, were collected. The presence of toxins will be investigated by the ELISA method. Blood and milk samples will be taken from Holstein cattle fed with this corn silage for a total of 90 heads, 2 cattle in each farm. Following the cytotoxic effect of mycotoxins in blood samples, secondary biochemical changes such as ALB, ALP, ALT, AMY, and BUN will be examined with an automatic analyzer. In addition, the presence of AFM1 (aflatoxin M1), which is the excretion form of aflatoxin in the milk of animals that consume aflatoxin-containing feeds, will be investigated by ELISA method in milk samples. AFM1 does not decompose in milk and dairy products, is highly resistant to different industrial processes such as heat treatments, sterilization, or pasteurization, and therefore it is important for public health.